Holmium Glass Reference for Wavelength in the UV/Visible
For assessing the wavelength accuracy of your spectrophotometer

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Starna Cells, Inc
PO Box 1919
Atascadero, CA 92423

Phone:
805-466-8855
800-228-4482 (toll free)

Fax:
805-461-1575

Email:
sales@starnacells.com

Description	Holmium glass filter
Primary Usage	Verify the wavelength scale of UV/Visible spectrophotometers
Useable Range	241nm to 640nm, instruments with SBW of less than 10 nm
Physical Configuration	Stress Free glass filter mounted in anodized aluminum holder
NIST Traceability	Each set is supplied with a Certificate of Analysis and NIST Traceability
ISO Accredited	Accredited by UKAS ISO 17025, ISO Guide34 and ISO 9001

Product Description

View/Download Comprehensive Data Sheet for Holmium Glass Reference

Holmium has been used as a wavelength reference for many years and like its liquid counterpart, the holmium glass filter produces characteristic peaks that make it suitable for use as a wavelength reference. As a solid reference material, the Starna holmium glass filter is more robust than the liquid cell as it is mounted in an anodized aluminum housing to offer greater protection.

Holmium offers sharp, stable peaks over the range of 241 nm to 640 nm.

The Starna holmium reference is used to assure that the wavelength scale of your instrument is within the manufacturers tolerances to the actual wavelength being measured. The use of the holmium glass reference filter assures that the optical configuration of your quality control is exactly the same as for a normal analysis.

Certified SBW and Wavelengths

SBW	nm	nm	nm	nm	nm	nm	nm	nm	nm	nm	nm
0.10	637.53	536.43	460.33	453.69	445.71	418.9	360.98	333.87	287.62	279.40	241.66
0.25	637.58	536.36	460.30	453.68	445.70	418.66	360.98	333.86	287.62	279.40	241.69
0.50	637.68	536.37	460.28	453.70	445.71	418.73	360.97	333.90	287.60	279.40	241.70
1.00	637.54	536.64	460.23	453.69	445.73	418.73	360.90	333.90	287.65	279.45	241.65
1.50	637.60	536.59	460.21	453.65	445.82	418.73	360.94	333.91	287.72	279.33	241.58
2.00	637.55	536.63	460.22	453.69	445.97	418.71	360.92	333.92	287.74	279.28	xxx.xx
3.00	637.51	536.87	460.42	453.58	446.21	418.86	360.94	333.95	287.82	278.82	xxx.xx

Note: xxx.xx indicates this peak may not be resolved at this SBW.

Suggestions for Use

Holmium presents a wide range of crisply resolvable peaks which are easily used to correlate the wavelength indicator on your spectrophotometer to the known Holmium peaks.

The initial procedure should be to scan the filter over the useable range to assure that all of the peaks can be resolved. Alter the slit width and scan rate to produce a useable spectra. Check each peak to assure that the reading on your spectrophotometer is within the manufacturer's tolerances for the wavelength of your spectrophotometer. If not, have a service technician check and, if necessary, adjust your instrument. Holmium is measured against an air blank.

Periodically check the spectra, at a minimum in the area where you are doing your analysis. The periodic use of your Holmium cell will build a log of your instrument's wavelength accuracy for use with certification and troubleshooting should the correlation change over time.

Absorbance Scan of Holmium Glass

Transmission Scan of Holmium Glass

Interpretation of Results

Consult your spectrophotometer's reference manual for the specification of your instrument's wavelength scale tolerance. This figure will be listed as a plus and minus (+/-) number in nanometers (nm). Add your instrument's wavelength tolerance to the confidence limit on the certificate supplied with the Holmium wavelength reference. Compare the peak wavelength values measured on your instrument with the expected values calculated from your instrument mannual and the NIST traceable certificate. If the readings are within the expected range for your instrument, then your wavelength scale is correct. If not, consult your service technician and have your instrument evaluated.

Quality program

Each time that the analysis is repeated you can compare the new results with the previous results to check for any variance. If your instrument needs service because of a change in resolution, the historical data will greatly assist the service technician.

How to Order

Catalog Number	Description
RM-HG	Wavelength Reference, Holmium Glass, NIST traceable and ISO 17025

Download MSDS Sheet for Holmium Glass Reference