Home Page Downloads How to Purchase Technical Assistance Starna Banner
Toluene in Methanol for derivative spectroscopy
Description 0.020% v/v solution of toluene in methanol, permanently sealed by heat fusion into a far UV quartz cell. Supplied with a methanol blank.
Primary Usage Resolve multiple peaks by calculating the derivative of the spectra
Physical Configuration Far UV quartz cells that have been permanently sealed by heat fusion
NIST Traceability Each set is supplied with a Certificate of Analysis and NIST Traceability
ISO Accredited Accredited by UKAS ISO 17025, ISO Guide34 and ISO 9001

Product Description

View/Download Comprehensive Data Sheet for Toluene in Methanol Reference

0.020% v/v solution of toluene in methanol, permanently sealed by heat fusion into a far UV quartz cell. Supplied with a methanol blank.

In derivative spectroscopy, absorption spectra aretransformed into first-, second- or higher-order derivatives.This technique facilitates the resolution of two or more peaksthat overlap and become merged into a composite curve, andare represented by shoulders on that curve rather than asindividual peaks. By calculating the derivative, the underlying individual spectra can be more readily identified. Probably themost used is the second order, i.e d2 A/dλ2.

Typical Spectra

When this reference material is scanned in 2nd derivative mode against the methanol blank, the resulting spectrum should be similar to that shown, with a small negative extremum at 285 nm between two larger negative extrema at 261 nm and 268 nm.


To meet the requirements of the Pharmacopoeia, the ratio A/B should be greater than 0.2:



How to Order

Catalog Number Description
RM-TM Resolution reference cell, Toluene in Methane with blank

Download MSDS Sheet for RM-TM Reference